Science Inventory

A Case Study: Using New Approach Methods to Assess Estrogen Receptor Activity

Citation:

Judson, R. A Case Study: Using New Approach Methods to Assess Estrogen Receptor Activity. Summer School 2020 Innovative Science Without Animals Virtual Meeting, RTP, NC, June 22 - 26, 2020. https://doi.org/10.23645/epacomptox.12988178

Impact/Purpose:

Presentation given to the the Summer School 2020 Innovative Science Without Animals – online virtual meeting – June 22 – 26, 2020. The program is focused on presenting cutting-edge methods to reduce and replace animal tests in toxicology and biomedical science.

Description:

The US EPA and other international regulatory agencies are required to evaluate certain chemicals for their ability to be “endocrine disruptors”, which includes testing for activity against the endocrine pathways, including the estrogen receptor (ER) pathway. The current testing approach at the EPA requires a large battery of in vitro and in vivo assays to fully test for ER activity. We have been exploring the use of high-throughput in vitro and in silico tests to supplement and partially replace some of the traditional low-throughput assays. This talk will describe the approaches we have developed for ER testing, including the ER IATA proposal submitted to OECD and a proposed defined approach (DA) for ER. This work does not reflect the official policy of the US EPA.